Urease preparation



Patented-July 31,1928.

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Io Drawing. Application illed September 8, 1927, Serial No. B17,527,and in Germany September 1988 This invention relates to a method-for making stable, easily soluble, light-colored and dry urease reparations from aqueous extracts of soya eans jack beansor similar parts of urease-contaming: vegetables.

The preparationpf urease, an enzyme decomposing urea, by extracting vegetables such as soya beans or jack beans with water is known. But extracts are' not stable and decompose ina short time; the albuminous substances precipitate and'i thus carry down the enzyme, thus 'weakeniugo'r. even annulling the etfectiveness ot'the "extract n decomposing urea. It .is' not possible vto stabilize aqueous extracts of the above mentioned kind b addition of salts, such as d1 -'If aqueous extracts of plants containing urease are eva orated at low temperatures eventually at re uced pressure a'dark-colored, horny mass is obtained, which is soluble in water. Furthermore by this evaporation a part of the urease is decomposed. But when the aqueous extract sodium-phosp ate.

is precipitated with alcohol or acetone and the precipitate is dried in known manner, a'

light-colored product is obtained, which is insoluble in water when it has been precipitated by alcohol and is solubleinwater when it has been precipitated by acetone.

By this precipitation however a great part of the urease isdestroyed by the substances used for preci itating'it and the products obtained there ore have varying degrees of atomizing such extract into eflectiveness. Y

The resent invention relates to a method forma ing'a stable-crease preparation not havin the above mentioned disadvantages. Accor ing to the invention an aqueous,

filtered and centrifugalized extract of soya beans, jack beans'or similar parts of plants containing urease is freed from water, by a steam of and heated air or gases. Althou h urease' in aqueous' solutions is decompose at temeraturesbetween 7 0 and 80 0., accord' mg to the invention temperatures for above be used, without injuring the 100 C. may

enzfime.

e product obtained according to the invention is of light color tine.-

and easily soluble. in water. It contains all the urease orig inally present and remains constantly effecf chemicals, f such as di-sodiumr phosphate, mono-potassium-phosphate or an mixtures of these substances, may be added to the aqueous extract of the urease or the plants may be extracted with solutions of these salts. The dry productsiobtained by atomization of these extracts into streams of slowlytherein, products containing urease 05f and the above mentioned salts-"and obtained by. atomization of extracts containing these. salts are quickly and easily dissolved in water.

Among the uses to which the product may V be put are for the analytical determination of urea, since urease will quantitatively decompose urea to ammonia; and as anaddit on to washing and detergent preparations whereby such preparations are made efiec- 7 tive to remove dirt from fire materials'without scrubbing or other rough treatment.

I claim: v

1. A. method for making stable, easily I soluble, light-colored, dry urease prepa-ra-J tions which consists in extracting" urease containing vegetables with water, adding water soluble phosphates to the aqueous'ex-' tract and atomiz'ing such extract into a dry and heated gas stream to free it from-watch 2. A method for making stable, easily soluble, light-colored, dry urease preparaq -t ions which consists in extracting ureasecontaining ve etables vvlth water, adding alkali-metal plosphates to the a ueous exj v9 tract and atomizing such extract into a dry I and heated as-stream to free it from water.

3; A met od for making stable, easily soluble, light-colored," dryurease p'reparations which consistsiii extracting urea'sed containin vegetables with aqueous solutions the resulting of phosp ates and atomizin extract into a dry and heate gas streamfto free it from water.

4. A .method for making stable, easily 1 6 soluble,- light-colored, dry ureasepreparations which consists inextracting 'urease containing vegetables with aqueous solutions 7 a of alkali-metal ,phosphates and atomizin the resulting extract into a dry and gas stream to free-it from water...

In testimony whereof I aflix m Sig an"; i- 

